Genome Annotation

We develop software and methods to improve genome annotations.

MacSyFinder, the macromolecular system finder

In particular we developed MacSyFinder, a software to:

  1. model molecular systems (pathways, machineries…) in microbial genomes
  2. annotate them in genomes

MacSyFinder takes advantage of the genomic organization by function of archaeal and bacterial genomes: genes participating in a same function, complex, pathway… tend to co-localize on the genome. This enables powerful, system-level (instead of gene-by-gene) annotation of functions.

For more details, have a look at the corresponding publication(s).

The MacSyFinder v2 search engine



Figure from Néron et. al 2023., the MSF v2 paper reviewed and recommended by PCI Genomics.

MacsyModels, an organization for sharing MacSyFinder models

Since MacSyFinder version 2, it is possible to easily download, install and share publicly available MacSyFinder models. This is done from the official MacSy-models organization. Several models are readily available, as described in Néron et. al 2023..

As of March 2023, Macsy Models included models for the detection of:

  • CRISPR-Cas systems CasFinder
  • bacterial protein secretion systems and type IV-filament super-family members TXSScan
  • conjugative systems CONJScan

For more on how to install MacSyFinder and the associated Macsy Models, have a look at the comprehensive MacSyFinder’s documentation.

Writing own MacsyModels

In the following publication, we explain step-by-step how one can write its own MacsyModels (models’ definition and profiles). If interested, have a look here:

Abby, Denise and Rocha 2024 Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder Version 2, 2024 https://link.springer.com/10.1007/978-1-0716-3445-5_1